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Image Search Results
Journal: PLoS ONE
Article Title: A Conditional Mouse Mutant in the Tumor Suppressor SdhD Gene Unveils a Link between p21 WAF1/Cip1 Induction and Mitochondrial Dysfunction
doi: 10.1371/journal.pone.0085528
Figure Lengend Snippet: A. SdhD mRNA levels in heterozygous (+/−) and SDHD-ESR adrenal medulla and kidney relative to wild-type (+/+) tissues 7 days after the start of the tamoxifen treatment, as obtained from the corresponding microarray feature (Gene Bank accession n° NM_025848). *, P ≤0.05; ***, P ≤0.001. The number of samples is 8 per group. B. Supervised hierarchical clustering of adrenal medulla (AM) and kidney samples based on genes that showed significant differences in their expression level. The heatmap and the hierarchical tree are shown for 8 samples, grouped in pairs, per genotype.
Article Snippet: For microarray analysis, 200 ng (kidney) or 45 ng (adrenal medulla) of RNA were reverse-transcribed into cRNA and labeled with the
Techniques: Microarray, Expressing
Journal: PLoS ONE
Article Title: A Conditional Mouse Mutant in the Tumor Suppressor SdhD Gene Unveils a Link between p21 WAF1/Cip1 Induction and Mitochondrial Dysfunction
doi: 10.1371/journal.pone.0085528
Figure Lengend Snippet: A. Comparative functional analysis of datasets generated from adrenal medulla (AM) and kidney samples from SDHD-ESR mice. The significance of each molecular and cellular function is indicated by –log of the p-value. B. Quantitative RT-PCR of RNA samples used in the microarray study based on specific primers for amplification of the Cdkn1a mRNA.
Article Snippet: For microarray analysis, 200 ng (kidney) or 45 ng (adrenal medulla) of RNA were reverse-transcribed into cRNA and labeled with the
Techniques: Functional Assay, Generated, Cell Function Assay, Quantitative RT-PCR, Microarray, Amplification